Rapid detection of avian leukosis virus subgroup J by cross-priming amplification

Abstract Avian leukosis virus subgroup J (ALV-J) causes oncogenic disease in chickens China, resulting great harm to poultry production, and remains widespread China. Herein, we employed a cross-priming amplification (CPA) approach nucleic acid detection device establish visual rapid method for ALV-J. The sensitivity of CPA, polymerase chain reaction (PCR) real-time PCR (RT-PCR) was compared, the three methods were used detect ALV-J cell cultures which inoculated with clinical plasma. result showed when carried out at 60 °C just min, CPA 10 times higher than conventional PCR, high specificity, comparable RT-PCR, based on 123 plasma, coincidence rate 97.3% (71/73). does not require an expensive instrument; simple water bath or incubator is sufficient complete DNA amplification, closed avoids aerosol pollution, making judgment results more intuitive objective. assay would be promising simple, sensitive identification